"comparative glutathione s-transferases (gsts) inhibition assay in the whole extract of fasciola hepatica and sheep liver tissue by hexachlorophene"
نویسندگان
چکیده
recent research highlights the importance of gsts in the establishment of chronic helminth infections. gsts have the potential to protect the parasite against the host immune response. in this present study, gst enzyme assay has been investigated on whole extract of f. hepatica and sheep liver tissue. to the 1-ml plastic cuvette, added 200 mm potassium phosphate buffer. then added 50 mm gsh reduced. was placed the required volume of f. hepatica or sheep liver extract into the cuvette and mixed well. added water and equilibrated at room temperature for 5 minutes. meanwhile was set up the uv spectrophotometer at 340 nm for the gst assay. finally was placed the cuvette into the barrel of the uv spectrophotometer and added 1-chloro-2, 4-dinitorbenzene (cdnb) and stirred well. for testing an inhibitor of gsts such as hexachlorophene, mixed the appropriate volume of compound with gsh,reduced buffer, water and protein before equilibrating at room temperature. for calculation of ic50% value a computer package was used. the inhibitor concentration for 50% remaining activity of gsts calculated graphically and wasobtained 10µl for f. hepatica and 20 µl for liver tissue.
منابع مشابه
"inhibition assay study of purified glutathione s-transferase from fasciola hepatica and sheep liver tissue by hexachlorophene"
glutathione s-transferases (gsts) are widespread in fasciola. hepatica parasite and sheep liver tissue. study of gsts inhibition assays in f. hepatica and sheep liver tissue are a priority of chemotherapeutic targets in parasitic liver diseases including human fascioliasis in iran. in this research, the whole extract of f. hepatica and sheep liver tissues were purified and eluted for sodium dod...
متن کاملInhibition Assay Study of Purified Glutathione S-transferase from Fasciola Hepatica and Sheep Liver Tissue by Hexachlorophene
Glutathione S-transferases (GSTs) are widespread in Fasciola. hepatica parasite and sheep liver tissue. Study of GSTs inhibition assays in F. hepatica and sheep liver tissue are a priority of chemotherapeutic targets in parasitic liver diseases including human fascioliasis in Iran. In this research, the whole extract of F. hepatica and sheep liver tissues were purified and eluted for sodium dod...
متن کاملcharacterization of purified glutathione s-transferase (gsts) from fasciola hepatica and liver tissue by two-dimensional electrophoresis (2-de)
two-dimensional electrophoresis (2-d electrophoresis) is a powerful and extensively used method for analysis of complex protein mixtures extracted from cells, tissue, or other biological samples such as helminth parasites including, f. hepatica. each spot on the resulting two-dimensional collection corresponds to a single protein species in the sample. this study was carried out to detect of gs...
متن کاملComparative assay of glutathione S-transferase (GSTs) activity of excretory-secretory materials and somatic extract of Fasciola spp parasites.
Fascioliasis is a worldwide parasitic disease in human and domestic animals. The causative agents of fascioliasis are Fasciola hepatica and Fasciola gigantica. In the recent years, fasciola resistance to drugs has been reported in the many of publications. Fasciola spp has detoxification system including GST enzyme which may be responsible for its resistance. Therefore , the aim of the study wa...
متن کاملstudy on glutathione s-transferase (gst) inhibition assay by triclabendazole. і: protoscoleces (hydatid cyst; echinococcus granulosus) and sheep liver tissue
hydatid disease is a term used to refer infection with the methacestode of echinococcus granulosus parasite in humans, and echinococcusis is restricted to infection with the adult stage in carnivores.glutathione s-transferase (gst) represents the major class of detoxification enzymes from helminth parasites such as echinococcus protoscoleces (psc) and it is candidate for chemotherapeutic and va...
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عنوان ژورنال:
iranian journal of public healthجلد ۳۰، شماره ۳-۴، صفحات ۱۲۵-۱۲۸
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